ATM Knockout cell line(22Rv1)

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ATM Knockout cell line(22Rv1) Order

ATM Knockout cell line(22Rv1)
Catalog#
YKO-H222
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
ATM Knockout cell line(22Rv1)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
ATM Knockout cell line (22Rv1)
Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:3
Species
Human
Gene Name
ATM
Gene ID
472
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90% RPMI-1640+10% FBS
Target region
Knockout region: Exon 3 (based on transcript ATM-229)
Validation: PCR+Sanger Sequencing
ATM Knockout cell line(22Rv1) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:22Rv1		 STR Info (Cell bank)
Cell Line:22RV1
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 1113111213.0
D13S317 912912
D7S820 91011.010119.0
D16S539 12121212
VWA 15211521
TH01 69.369.3
AMEL XYXY
TPOX 8888
CSF1PO 10111011
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the ATM gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the 22Rv1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, ATM gene knockout ATM Knockout cell line (22Rv1) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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